Welcome! This page is intended for collaborators participating in the second phase of the Earth Microbiome Project — the metagenomic sequencing and metabolomic profiling of five hundred microbial communities from diverse sites on our planet — informally called the EMP500. If you are not currently involved in this effort but are interested in contributing samples in the future, please contact Dr. Luke Thompson for more information.

In preparation for this new EMP effort, we are developing new protocols for metagenomic sequencing and assembly, with the goal of applying this workflow to a range of environmental samples, combined with metabolomic profiling. Our goal is to assemble a set of ~500 fresh environmental samples across a range of habitats, with the help of the EMP network of collaborators. We have funding to do metagenomic sequencing, assembly-free and assembly-based analysis, and metabolomics of these 500 samples. A biobank of frozen aliquots for all samples will also be maintained at UCSD (soil samples will be held at PNNL) for future methods testing and analysis.

Samples for this study will ideally meet the following criteria:

  • Fresh or frozen sample (no preservatives) in ~10 aliquots
  • Metadata well characterized
  • Large proportion of unmapped diversity in at least some samples
  • Non-human-associated and non-human-perturbed samples will be given priority

Detailed instructions for submitting samples and preparing metadata are here: